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REFLECTION ON THE CONTEXT OF KEY COMPETENCES OF STUDENTS IN TEACHING CELL BIOLOGY

 

Dr. Isa Hadjiali, Assist. Prof.

Department of Biology Education

Sofia University “St. Kliment Ohridski”

 

Dr. Vasiliki Adamoula

1st Lyceum of Elassona

 

Dr. Petar Raychev, Assist. Prof.

Faculty of Medicine

Sofia University “St. Kliment Ohridski”

 

Dr. Nadezhda Raycheva, Assoc. Prof.

Department of Biology Education

Sofia University “St. Kliment Ohridski”

 

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Ο ΠΡΟΒΛΗΜΑΤΙΣΜΟΣ ΣΤΟ ΠΛΑΙΣΙΟ ΤΩΝ ΒΑΣΙΚΩΝ ΙΚΑΝΟΤΗΤΩΝ ΤΩΝ ΜΑΘΗΤΩΝ ΣΤΗ ΔΙΔΑΣΚΑΛΙΑ ΤΗΣ ΒΙΟΛΟΓΙΑΣ ΤΟΥ ΚΥΤΤΑΡΟΥ

 

Dr. Isa Hadjiali, Assist. Prof.

Department of Biology Education

Sofia University “St. Kliment Ohridski”

 

Dr. Βασιλική Αδαμούλα

1ο Λύκειο Ελασσόνας

 

Dr. Petar Raychev, Assist. Prof.

Faculty of Medicine

Sofia University “St. Kliment Ohridski”

 

Dr. Nadezhda Raycheva, Assoc. Prof.

Department of Biology Education

Sofia University “St. Kliment Ohridski”

 

 

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Abstract. The article presents the idea of how the reflection educational tasks can serve as a means of formation and development of key competences for 16-17 year old students. The structure of the test is presented and it consists of two parts. The first part includes eight tasks that require students to identify scientific problems, scientific explanation of natural processes and phenomena and how to use scientific data and evidence. The second part consists of four questions requiring students to reflect how to solve problems from the first part of the test. All of the study results were processed by descriptive statistics, Spearman–Brown’s coefficient and Pearson coefficient.

 

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INTRODUCTION

 

Over the last years of the XX and the early XXI century, reflection has become a topical study problem area of both regional and international levels (Loughran, 2002; Stephens & Winterbottom, 2010). The majority of researchers pointed out that the main reason we should look for, is the positive functions of reflection on the overall educational process. On the one hand, it stimulates the intellectual and the overall cognitive development of the individual, on the other hand, it promotes his socialization in the modern globalized world (Shambaugh & Magllaro, 2001; Thorpe, 2000; Zohar & David, 2008; Zohar & Peled, 2008).

We can reasonably claim that the reflective issue has got serious traditions not only as an instructive theoretical line but also as theoretical and empirical psychological, pedagogical and methodological research. The majority of authors note that reflection is „…a psychological procedure or a process which is consciously directed and gives meaning to self-knowledge - the knowledge of somebody’s cognitive activity..“ (Kolarova-Kancheva, 2003; Vasilev et al., 2005). As a compact and complex psychological process it exists and manifests itself in several different modes - intellectual, personal, communicative and cooperative (Semenov & Stepanov, 1983). Although the reflective modes classificational framework is almost entirely artificial, abstraction of its defining characteristics support its formation, development and diagnostics purposefully. Immanent inherent, the intellectual reflection is the most relevant to the educational process. The classic in the field of reflective problems J. Dewey describes the reflective thinking as "an active , urgent and careful consideration of  any opinion or supposed form of knowledge in the light of the grounds that it confirms and further deductions to which it leads" (Dewey, 1997). J. Piaget examines the reflection (the reflective abstraction) as ability of the subject to separate and become aware of its cognitive actions to analyze not only the object and the result but also the means by which it is achieved (Piaget, 1977). Intellectual reflection is regarded as "an awareness on the grounds of our thoughts sources, actions and knowledge ... a reproduction of the ways and means by which the knowledge is gotten" (Kolarova-Kancheva, 2003; Kosolapov, 1983; Vasilev et al., 2005).

European Qualifications Framework (EQF) for lifelong learning and the European Reference Framework for key competences (ERFKC) accept as basic by its very nature normative documents for the development of the educational system (Raycheva & Tzanova, 2012; Tzanova & Raycheva, 2012). In this context, reflection can be highlighted as essential in the structure of key educational competences of the learner - communication in the mother tongue, communication in foreign languages, mathematical competence and basic competences in science and technology, digital competence, learning to learn, social and civic competences, sense of initiative and entrepreneurship, cultural awareness and expression (Lassnigg & Mayer, 2001; Otten & Ohana, 2009).

The foundation of the modern European educational space is not applied exclusively to solid scientific factual knowledge, but to implementation of educational programs that aim to develop competencies, cross-curricular integration and active learning, reflection, etc. The various European countries aim to develop key competences in two ways: - inclusion as a state educational standard in the curriculum (Austria, Finland, Germany, UK, Czech Republic, etc.) or changes in educational legislation (Belgium, France, Italy, Spain, Portugal and others.) (Boiadjieva et al., 2011).

At this stage the international comparative research in the field of school education as TIMSS (Trends in International Mathematics and Science Study) and especially PISA

(Program for International Student Assessment) gives the main source of information about the current status and the extent of formation of key competence. The international measurement of key competencies results in Mathematics and the Natural Sciences in Bulgaria, report some disturbing trends: first - the achievements of Bulgarian students compared with their peers from Europe and other countries such as China, Japan, Singapore, etc. are lower; second - students are not motivated for learning. We emerge similar negative trends in some national studies in the natural sciences (Kirova et al., 2010; Tafrova-Grigorova et al., 2009).

 

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MATERIALS AND METHODS

 

The main objective of the present study is a development of a set of learning tasks for reflection as a means of formation and development of key competences for 16-17 year old students in school training in cell biology. The sample includes 50 students studying cell biology as a compulsory selective training, section "Microsystem”- structure and processes.

The main idea of the investigation is the educational tasks understanding for reflection as a means of formation and development of student key competences. They are made to promote the activities of reflection in students over their own cognitive activity in the absorption of selected thematic content. A characteristic feature of these tasks (labeled provisionally by us with the term "reflective tasks") is the deliberate creation of problematic situations that require the learner to reflect on the grounds of their knowledge on his own cognitive actions to achieve the final result. They are made to promote the reflection activities of students over their own cognitive activity in the absorption of selected thematic content. A characteristic feature of these tasks (indicated provisionally by us with the term "reflective tasks") is the deliberate creation of problematic situations that require the learner to think over the grounds of his knowledge and over their own cognitive actions to achieve the final result. Each of these tasks aims to realize how the learner made his decision rather than to monitor the right result.

Achieving the objective requires constructing a reliable research tool which has to perform not only diagnostic but also developing role in training. In relation to those mentioned above there is a test, which consists of two parts.

The first part includes eight tasks that simulate problematic situations that promote reflexive reasoning of students over the grounds and the role of their knowledge and actions in their application within the particular specific situation (biological themes). In the key competences context tasks students have to establish scientific problems, scientific explanation of natural processes and phenomena using scientific data and evidence (Appendix 1). The arrangement of the elements of the situation is realized in terms of illuminating the choices that focuses on:- the transmission and application of knowledge; - analysis of the described situation; - analysis of elements, relations. The arrangement of the elements of the situation is realized in terms of illuminating the choices that focuses on structures which helps the formation of conclusions - information conversion - a synthesis of facts and reorganization of information and activities (Internal Feedback 1 IF 1).

The second part consists of four questions, directing to comparison basis of the inner feedback of the first part and argumentation towards the goal by choice/or by an individual answer. Thus questions direct the student to reflect how- in what way the tasks of the first part can be solved. (Internal Feedback 2 IF 2). This added "reflective end" aims not only to make students think over the similarity and the difference of these answers but also to inform the teacher and the student about the achieved results after the biology course (Fig. 1).

 

Fig. 1.

 

The main objectives of the first part of the tests are to show the ability of pupils to:

·         Task 1. Estimate the available information related to the significance of the presented experiment proving the genetic role of DNA;

·         Task 2. Structure information reflecting significant signs of aerobic biological oxidation and photosynthesis;

·         Task 3.  Prognosticate on possible changes in the DNA structure as a result of specific factors impact;

·         Task 4. Apply the basic principles of cell theory as analyze the results of an experiment;

·         Task 5. Transform information reflecting consequential connections between cell structures and processes, illustrating the application of the recombinant DNA technology from a scheme into a text;

·         Task 6. Reveal the relations between information items characterized consequential connections between cell structures involved in the implementation process of replication;

·         Task 7. Structure information reflecting significant signs of malignant transformed and normal human cells;

·         Task 8. Summarize the given information related to the application of monoclonal antibodies in modern immunology as short judgments.

 

RESULTS AND DISCUSSION

 

The analysis of the results in the first part of the test shows (Fig. 2) that the easiest task is No 7.This requires students to structure information reflecting significant signs of malignant transformed and normal human cells (task 7: 8% wrong partly correct 28%, completely correct 64%). According to the students answers the most difficult task is No 8. In terms of its content, students have to summarize information related to the application of monoclonal antibodies in modern immunology as short judgments (task 8: wrong 28% partly correct 60%, completely correct 12%).

 

Fig. 2. A presentation of the test tasks answers in percentage.

 

The students responses to questions №1 and №2 of the second part of the test (Table I) confirm the above mentioned percentage. Students declare that they did their best in task 7 (18 students - 36%) and task 2 (13 students - 26%), which require students to structure information reflecting significant signs of aerobic biological oxidation and photosynthesis.

 

TABLE І. Success and failure in tasks solving.

 

of tasks

Question 1.

Which one of the test tasks you did the best?

Question 2.

Which one of the test tasks was the most difficult?

 

№ 1

5

(10%)

22

(44%)

 

№ 2

13

(26%)

8

(16%)

 

№ 3

10

(20%)

17

(34%)

 

№ 4

6

(12%)

21

(42%)

 

№5

15

(30%)

8

(16%)

 

№6

16

(32%)

10

(20%)

 

№7

18

(36%)

9

(18%)

 

№8

3

(6%)

18

(36%)

Note - the percentages exceed 100%, as the majority of students pointed out more than one possible answer.

 

 

TABLE ІІ. Question 3 and question № 4 answer results.

 

Question 3. How did you manage to solve the given problems?

By applying the acquired knowledge of basic biological concepts and laws.

12

(24%)

Via resourcefulness and logic you searched about as you were solving the problem.

14

(28%)

Via your classmates help.

2

(4%)

Via intuition and guessing the correct answer.

5

(10%)

By using your own abilities and acquired skills.

17

(34%)

Question 4. What skills do you think that you need to achieve a complete success in solving similar tasks?

How to formulate guesses (hypotheses).

16

(32%)

How to justify and prove allegations.

12

(24%)

How to analyze connections and dependencies between individual parts.

5

(10%)

How to summarize and formulate conclusions.

7

(14%)

How to detect common principles as you solve some problems.

10

(20%)

 

As for the most difficult task in the test, students pointed out task 1 (22 students - 44%). The task aim is to measure the ability of the student to estimate the available information related to justifying significance of the presented experiment proving the genetic role of DNA. The second one is task 4 (21 students - 42%), which requires students to apply the basics of cell theory in analyzing the results of an experiment.

The analysis of the students’ answers to a question №3 is the basis for these analysis and comments. 34% (17 students) declare that they have overcome the difficulties which have arisen by using their own abilities and acquired skills. The second most common answer according to the percentage is from (14 students - 28%) who responded that the main way to overcome the difficulty in the process of resolving a task is to search resourcefulness and logic. As regards the question №4 results, namely the necessary skills to solve similar tasks illustrate that 16 respondents (32%) think that they should learn how to formulate assumptions (hypotheses), second (12 - 24%) students choose skills on how to justify (justify) and prove the allegations and the third (10-20%) skills to detect common principles as you solve some problems (TABLE II).

 

TABLE III. Correlation analysis.

 

 

Correlation coefficient

Correlation between the results in the first part of the test and the answers to the question №3

 

Spearman–Brown’s coefficient (rsb)

Correlation Coefficient = (0,602**)

p=0,000

p < 0,01

N= 50

H0- is rejected

 

Pearson coefficient (r)

Correlation Coefficient = (0,685**)

p=0,000

p < 0,01

N= 50

H0- is rejected

 

The conducted correlation analysis is connected with the empirical verification of the following statistical hypotheses:

Null hypothesis (H0): Between variables X and Y, which describe with numerical values the achievements of the students in the first part of the test and the answers to the question №3, there isn’t any significant positive correlation.

Alternative hypothesis (H1): Between variables X and Y, which describe with numerical values the achievements of the students in the first part of the test and the answers to the question №3, there is a significant positive correlation.

For verification of statistical hypotheses nonparametric methods were used to calculate the correlation coefficient: Spearman-Brown's coefficient (rsb) and Pearson coefficient (r).

The correlation analysis clearly shows that there is a significant correlation between variables X и Y, which describes with numerical values the achievements of the students in the first part of the test and the answers to the question №3. (rsb=0,602, p=0,000, p < 0,01; r =0,685, р=0,000, p < 0,01) (TABLE III).

The empirical values of SpearmanBrowns coefficient (rsb) and Pearson Correlation (r). Show a significant correlation between the two investigated signs. They are a sufficient reason to accept the alternative hypothesis (H1): Between variables X and Y, which are described with numerical values the achievements of the students in the first part of the test and the answers to the question №3, there is a significant positive correlation.

 

 

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CONCLUSION

 

The results of this study can be used as a basis for the reflections and generalizations in at least three interconnected boards:

1. The two reference frameworks, European Qualifications FrameworkQF) for lifelong learning and European Reference Framework for Key Competences (ERFKC ) outline the field of the social contract of the European Education in which area the reflection is an inseparable part of the personal and professional development. In this sense the formation of reflexive skills is of a legislative context.

2. The formation and development of reflexive skills is a goal as in the context of the relationship "subject - object" in view of the quality of the final product, so in the relationship "subject - subject" in view of the personal qualities of the subjects. A specific constructed task is an effective instrument in the sense of that aim.

3. The specificity of the reflexive tasks includes as a component of the task, such an internal feedback functioning mechanism which on one hand is the source for the determination of the parameters of a future corrective action and on the other hand for evaluating (by the teacher) of the formational level of the reflexive skills and self-assessment of the students.

 

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REFERENCES

 

Boiadjieva, E., Kirova, M., Tafrova-Grigorova, A. & Hollenbeck, J. (2011). Science learning environment in the Bulgairan school: student’s beliefs. Chemistry, 20 (1), 43 – 56.

Dewey, J. (1997). How we think. Mineola. New York: Dover Publications Inc.

Kirova, M., Boiadjieva, E. & Tafrova-Grigorova, A. (2010). Students’achievments approve the state educational requirements. Chemistry, 19 (2), 116 – 140.

Kolarova-Kancheva, T. (2003). The intellectual reflection in the training in Biology, grade 9. PhD thesis (in Bulgarian). Plovdiv.

Kosolapov, V. (1983). The reflection in the framework of the scientific hypothesis.  In: – The reflection, issues in the scientific knowledg (in Russian), Kuybyshev, Kuybyshev State University, pp. 133-138.

Lassnigg, L. & Mayer, K. (2001). Definition and selection of key competencies in Austria. Viena, Equihs.

Loughran, J. J. (2002). Effective reflective practice: in search of meaning in learning about teaching. J. Teacher Education, 53 (1), 33 – 43.

Otten, H. & Ohana, Y. (2009). The eight key competencies for lifelong learning: an appropriate framework within which to develop the competence of trainers in the field of European youth work or just plain politics? IKAB.

Piaget, J. (1977). Recherches sur l,absraction reflechissante. Paris, PUF, v.1-2.

Raycheva, N. & Tzanova, N. (2012). Methodology of biology education. Tasks for the formation of professional competencies of teacher. Sofia, Pensof (in Bulgarian).

Semenov, I. & Stepanov, S. (1983). The problem about the subject and methods of psychological studying of reflection. In: – Research of the problems in the psychology of creativity, Moscow, pp. 154 – 182, (in Russian).

Shambaugh, N. & Magllaro, S. (2001). A reflexive model for teaching instructional design. Educational Technology Research and Development, 49 (2), 69 – 92.

Stephens, K. & Winterbottom, M. (2010). Using a learning log to support students’ learning in biology lessons. Journal of Biological Education, 44 (2), 72-80.

Tafrova-Grigorova, A., Boiadjieva, E., Kirova, M. & Kuzmanov, A. (2009). External evaluation on the students’achevements: chemistry and environment – 9th grade. Chemistry, 18, 94 – 123.

Thorpe, M. (2000). Encouraging students to reflect as part of the assignment process: student responses and tutor feedback. Active Learning in Higher Education, 1 (1), 79–92.

Tzanova, N. & Raycheva, N. (2012). Methodology of biology education - theory and practice. Sofia, Pensof (in Bulgarian).

Vasilev, V., Dimova, Y. & Kolarova, T. (2005). Reflection and education. Part I. Reflection – theory and practice. Plovdiv, Macros (in Bulgarian).

Zohar, A. & David, A. B. (2008). Paving a clear path in a thick forest: a conceptual analysis of a metacognitive component. Metacognition Learning, 4, pp. 177 – 195.

Zohar, A. & Peled, B. (2008). The effects of explicit teaching of metastrategic knowledge on low - and highachieving students. Learning and instruction, 18 (4), 337-353.

 

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APPENDIX 1.

 

Task 1. 3. The text reflects the data for a scientific experiment carried out by a British biologist Fred Griffith, based on the information contained in the text and Figure 1, post suggestion for the purpose of the experiment.

 

& Text:

In 1923, Fred Griffith experimented with two strains of pathogenic and non-pathogenic Pneumococci. The pathogenic pneumococci are coated with mucosal polysaccharide capsule which is essential for the manifestation of their pathogenicity. These bacteria form smooth colonies and therefore are referred to as S- colonies. The mutant strains are deprived of their polysaccharide capsule and are not pathogenic. They form irregular colonies, therefore are referred to as R.

In his experiments F. Griffith injected mice with a mixture of alive non-pathogenic (R) and dead bacteria (S) (killed by heating, that caused their death). According to Griffith, the cause of death of the mice was the existence of an "active" compound in pathogenic cells that remains intact under the heat treatment and later were transferred to the non-pathogenic pneumococci, transformed them into pathogenic.

http://study.com/cimages/multimages/16/Griffith_1928a.png

Fig. 1.  Fr. Griffith experiment.

 

А. Griffith proved the transformation function of DNA to converse of non-pathogenic to pathogenic pneumococci.

B.  He proved the role of mice as a target for genetic researches.

C. He proved the genetic role of DNA for storage and transmission of the hereditary program.

D. He proved the role of mutations on living organisms.

E. He proved the morphological characteristics of both strains of pneumococci.

¤ In the answer sheet circle the letter of the answers that are most appropriate.

 

Task 2. In items A - E there are five statements about the aerobic process of the biological oxidation and photosynthesis. Define only one question that requires bringing together of all the above mentioned statements.

А. In the process of photosynthesis the light energy is used for the conversion of the low energy oxidized form of carbon (CO2) at a high energy reduced form of C in the composition of the organic compounds. The aerobic biological oxidation is the final stage of the degradation of the organic substances, which then release a significant amount of energy (ATP) to form H2O.

B. In the light phase of photosynthesis, electrons hit by the pigment molecules are transported through electron chains to coenzyme (NADP) and the released energy is used for the synthesis of ATP. At the biological oxidation, electron transfer to the final acceptor (O2) is carried out through intermediate transmitters (respiratory chain), resulting in the release of energy for synthesis of ATP.

C. The sources of electrons in photosynthesis are pigment molecules SNS, which are activated by absorbance of light quanta. Biological oxidation source of electrons is the oxidizing substrate.

D. In the process of photosynthesis, besides synthesis of ATP during electron transportation, the reduction of NADP is carried out too. In organic oxidation consistent electron transfer is performed by reduced output NAD (NAD. H2) to the final oxidizer O2 to form H2O.

E. In photosynthesis, the conversion of the light energy into energy of ATP requires the participation of a special ATP-synthase complex located in the membranes of the thylakoids of the chloroplasts. In organic oxidation, the transformation of the released energy in the respiratory chain into energy of ATP is associated with the activity of ATP synthase complex, produced in the inner mitochondrial membrane.

! Provide your reasoning in the answer sheet.

 

Task 3. Suggest and justify at least a hypothesis about the possible changes in the structure of DNA if:

û А. DNA was heated at 85оС- 100оС.

û Б. A mixture of single-stranded DNA fragments isolated from human and chimpanzee are placed at 65 о С.

! Provide your reasoning in the answer sheet.

 

Task 4. In Figure 2 consecutive stages of an experiment conducted with Rana ridibunda are presented. Analyze the scheme. Which one of the listed in Item. A - E positions of the cell theory should you use as an argument to explain the results?

 

Fig. 2. Experiment with Rana ridibunda.

 

 

А. The cell is the smallest structural and functional unit of living matter.

B. Cells of the different organisms are similar (homologous) in structure.

C. Cells are reproducted by division.

D. Multicellular organisms are complex mixtures of cells, united in a uniform system of tissues and organs connected through neural and humoral regulation.

E. All cells in a multicellular organism are totipotent, i.e. each cell carries the genetic information for the development of the whole organism.

¤ In the answer sheet circle the letters of the answers that are the most appropriate.

 

Task 5. Write a short text, that reflects the relationship between the reflected structures and processes in Figure 3, illustrating the application of recombinant DNA technology for industrial production of the insulin hormone.

 

Fig. 3. Human insulin production.

!. Write your text (not more than 5-6 sentences) in the answer sheet.

 

Task 6. Write a short text that reflects the connection between the listed in Item. A to H. components of the cell and their participation in the implementing process of replication.

 

А. DNA matrix                                               E. topoisomerase

B. activated deoxyribonucleotides                  F. helicase

C. replicative fork                                           G. DNA-polymerase

D. Okazaki fragments                                     H. DNA ligase

! Write your text (not more than 5-6 sentences) in the answer sheet

 

Task 7.  In Item. A - E five statements about cancer cells are given. Define only one question that requires a reasoned reply to the unification of all these statements.

А. Cancer cells divide continuously for unlimited period of time, which explains why their number is growing rapidly. In a fast-growing tumor with a diameter of 0.5 cm. there are a few billion of cells. The abnormalities in their genetic program are transmitted to the division daughter cells.

B. Normal cells growing in a cell culture form a monolayer that covers the walls of the vessel and after that, division stops. Unlike normal cells, cancerous cells do not possess this property. They divide as long as conditions exist (nutrients, oxygen, optimal temperature) forming a shapeless cellular mass.

C. Cancer cells are not specialized. They appear obvious morphological differences comparing with the normal cells of the same tissue. Cancer cells of the skin, for example, are more rounded and soft comparing with the flat and solid cells of the epidermis.

D. Cancer cells penetrate between the cells of other tissues or in the blood flow and form elsewhere in the body tumor masses (metastasis). This is one of the reasons for the difficulty of treating cancer at a later stage of the disease.

E. Carcinogenic cells can easily be transplanted into another organism where they continue to divide. A transplant of a carcinogenic cell in a healthy animal after a certain time causes cancer.

! Write the question in the answer sheet.

 

Task 8. The text gives information for obtaining monoclonal antibodies from cell cultures. Which of the following in Item. A - E data can be used as an argument to demonstrate the practical application of this method in modern immunology?

&Text:
Antibodies are produced by a special type of white blood cells, the B-lymphocytes. If you isolate a single B-lymphocyte (a branch) from an immunized animal you will isolate cells with a specific antibody. These cells, however, have a short life expectancy and cannot be cultivated in a nutrient medium. That's why for the preparation of monoclonal antibodies a technique is applied , also known as the “hybridoma technique”.

 This technique consists in the following: in an appropriate medium a cell fusion is performed between two types of cells, B -lymphocytes with a cancer antigen from an immunized animal (usually a mouse) and transformed B-lymphocytes (known as myelomas). The resultant hybrid cell is an hybridoma, that has inherited the ability to synthesize antibodies with a specific antigen from B-lymphocytes and the ability for an unlimited division and cultivation from the myeloma.

 

¤ Circle the letter of the answers you consider to be the most appropriate in the answer sheet:

А. The data for the ability of the hybridoma cells to produce antibodies with a predetermined antigenic specificity.

B. The data for the production of hybrid cells - hybridomas.

C. The data for the isolation of the fusion antigen specific antibodies

D. The data for the composition of the culture medium in which the hybridomas are cultured.

E. The data for the ability of hybridoma cells for an unlimited division and cultivation.

 

 

 

 

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